From Bandai Namco:
As an end-game challenge for players, Deep of Night first starts by hiding the Nightlord and any Shifting Earth from players until battle begins. Enemies across Limveld will be more challenging and random enemies will be mutated and present extreme danger to players. To help overcome the newly strengthened foes, new weapon variants and enhancements will be available for players to discover, but these could also contain a detrimental effect such as increasing damage taken as well. Being highly selective will be necessary for surviving through the night and defeating the Nightlord. Players will be able to unlock three new relic slots exclusively for Depths Relics, a new reward players can earn in Deep of Night mode. These Depths Relics can add powerful enhancements for players, but like the new variant weapons, the power also comes at a cost for players. Skillful calculation and execution will be necessary to be victorious. Deep of Night mode is for the bravest Nightfarers, providing extreme challenge, showing them the darkest depths of the game, even the possibility of unknowingly facing an Everdark Sovereign Nightlord.